DNA recovery after sequential processing of latent fingerprints on black polyethylene plastic.

Latent fingerprints on plastic substrates can be visualized by using sequential treatments to enhance the contrast between the fingerprint residues and underlying substrate; however, the extent to which these processes affect subsequent DNA analysis is mostly unknown. Latent fingerprints deposited on black plastic by one donor were visualized with single-process fingerprint powders (i.e., white powder, bichromatic powder, or bichromatic magnetic powder) or sequential treatments (i.e., laser → reflected ultraviolet imaging system (RUVIS) → CA fuming → RUVIS → Rhodamine 6G, Ardrox, and MBD (RAM) or CA fuming → RAM/laser → bichromatic magnetic powder). Samples were examined after the addition of each treatment. DNA was collected using cotton swabs, extracted, quantified, and amplified. DNA yields, peak heights, number of alleles obtained, and percentage of DNA profiles eligible for CODIS upload were examined. Latent fingerprints processed with the laser and up to three sequential treatments generated DNA profiles with significantly higher peaks heights than those of the untreated samples. Fingerprints processed with the laser and up to two sequential treatments generated DNA profiles with significantly more alleles. All methods beginning with laser enhancement generated more CODIS-eligible profiles. Additional research is needed to determine the extent to which initial laser enhancement impacts the success of downstream DNA profiling results. Although DNA profile development is not guaranteed due to the variable quantities of DNA contained within latent fingerprints, the selection of an appropriate latent fingerprint visualization method could maximize both fingerprint detection and the generation of CODIS-eligible DNA profiles.

DNA recovery after sequential processing of latent fingerprints on copy paper.

Forensic examiners must determine whether both latent fingerprint development and DNA profiling can be performed on the same area of an evidence item and, if only one is possible, which examination offers the best chance for identification. Latent fingerprints can be enhanced by targeting different components of fingerprint residues with sequential chemical treatments. This study investigated the effects of single-reagent and sequential latent fingerprint development processes on downstream DNA analysis to determine the point at which latent fingerprint development should be stopped to allow for DNA recovery. Latent fingerprints deposited on copy paper by one donor were developed using three sequential processes: 1,8-diazafluoren-9-one (DFO) â†’ ninhydrin â†’ physical developer (PD); 1,2-indanedione-zinc (IND-Zn) â†’ ninhydrin â†’ PD; and IND-Zn â†’ ninhydrin â†’ Oil Red O (ORO) â†’ PD. Samples were examined after the addition of each chemical treatment. DNA was collected with cotton swabs, extracted, quantified, and amplified. DNA yields, peak heights, number of alleles obtained, and percentage of DNA profiles eligible for CODIS upload were examined. DNA profiles were obtained with varying degrees of success, depending on the number and type of treatments used for latent fingerprint development. The treatments that were found to be the least harmful to downstream DNA analysis were IND-Zn and IND-Zn/laser, and the most detrimental treatments were DFO, DFO/laser, and PD. In general, as the number of treatments increase, the opportunities for DNA loss or damage also increase, and it is preferable to use fewer treatments when developing latent fingerprints prior to downstream DNA processing.

Silver Nitrate Grade and Its Effect on Physical Developer Performance-A Validation Study.

Silver nitrate is a critical component of the physical developer (PD) reagent. Significant increases in the cost of silver nitrate in recent years have caused the United States Secret Service's Forensic Laboratory to look for a more economical way to produce physical developer. One possible solution to this dilemma is to use a lower grade of silver nitrate, which typically has a lower cost. This study compared the quality of fingerprints produced on various paper substrates by three physical developer working solutions, each prepared using either the American Chemical Society (ACS), U.S. Pharmacopeia (USP), or technical grade of silver nitrate. It was determined that the less pure grades of silver nitrate produced approximately the same quality of fingerprints as the ACS grade, which is currently used in the authors' laboratory for making PD working solutions. In the experiments comparing PD prepared using the ACS and USP grades of silver nitrate, the ACS grade was superior only 5% of the time, whereas the USP grade was chosen 27% of the time. In the experiments comparing PD prepared using the ACS and technical grades of silver nitrate, the ACS grade was superior only 10% of the time, whereas the technical grade was selected 17% of the time. For the majority of the sample comparisons, no difference in fingerprint quality was observed. The overall conclusion was that either USP or technical grade silver nitrate can be used in place of the current, and more expensive, ACS grade of silver nitrate.

Comparison of the columnar-thin-film and vacuum-metal-deposition techniques to develop sebaceous fingermarks on nonporous substrates.

Both the columnar-thin-film (CTF) and the vacuum-metal-deposition (VMD) techniques for visualizing sebaceous fingermarks require the deposition of a material thereon in a vacuum chamber. Despite that similarity, there are many differences between the two techniques. The film deposited with the CTF technique has a columnar morphology, but the film deposited with the VMD technique comprises discrete islands. A split-print methodology on a variety of fingermarked substrates was used to determine that the CTF technique is superior for developing fingermarks on clear sandwich bags and partial bloody fingermarks on stainless steel. Both techniques are similar in their ability to develop fingermarks on glass but the CTF technique yields higher contrast. The VMD technique is superior for developing fingermarks on white grocery bags and the smooth side of Gloss Finish Scotch Multitask(™) tape. Neither technique worked well for fingermarks on black garbage bags.

Use of an optimized 1,2-indanedione process for the development of latent prints*.

1,2-Indanedione belongs to a class of compounds which have demonstrated great potential in the processing of latent prints, particularly in the area of fluorescence. However, variability in results achieved worldwide has precluded it from being used extensively. In order to isolate the cause of this variability, various components of the formulation were analyzed, including purity level of the indanedione, type of carrier solvent, and the use of ZnCl(2) both as a secondary application and incorporated into the reagent. Using a resultant optimized formulation (Ind-Zn), performance comparisons were then made in the areas of visible color development, fluorescence, and degree of substrate staining with those of 1,8-diazafluoren-9-one (DFO) for both fresh and aged prints. Moisture content of the paper substrates on which the prints had been deposited was measured and a correlation found with percentage ambient relative humidity (% RH). Determination of visible color and fluorescence as it corresponded to percentage moisture content allowed for defining critical threshold levels necessary for achieving optimal results. Correlating these values with % RH then allowed for the development of standard operating procedures for obtaining best possible print development. Through this work, it was determined that a 7.4% v/v formulation of Ind-Zn having petroleum ether as a carrier solvent yielded the most optimal results when processing methods optimized for % RH in the laboratory were utilized. Both initial color development and fluorescence were superior to that of DFO; prints developed with Ind-Zn were a minimum of 6.5 units dE* darker and more red than with DFO for all substrates tested. Processing with Ind-Zn on the majority of the substrates examined yielded fluorescence intensity values approximately four times greater than with DFO.

Effect of electron beam irradiation on forensic evidence. 2. Analysis of writing inks on porous surfaces.

The effect of electron beam irradiation on a series of different writing inks is described. As the anthrax-tainted letters were discovered in October 2001, the U.S. government began to experiment with the use of the electron beam irradiation process for destroying such biological agents. Plans initially considered a large-scale countrywide use of this technology. However, over time the scope of this plan as well as the radiation dosage were reduced, especially when some adverse consequences to mailed items subjected to this process were observed. Little data existed at the time to characterize what level of damage might be expected to occur with common items sent through the mail. This was especially important to museums and other institutions that routinely ship valuable and historic items through the mail. Although the Smithsonian Institution initiated some studies of the effect of electron beam irradiation on archived materials, little data existed on the effect that this process would have on forensic evidence. Approximately 97 different black, blue, red, green, and yellow writing inks were selected. Writing ink types included ballpoint, gel, plastic/felt tip, and rollerball. All noncontrol samples were subjected to standard mail irradiation conditions used by the U.S. Postal Service at the time this experiment was performed. A video spectral comparator and thin-layer chromatography (TLC) analysis were used to evaluate both the control and the irradiated samples. Some published studies reported changes in the presence/absence of dye bands in the chromatograms of irradiated writing inks. Some of these studies report the formation of additional dye bands on the chromatogram while others report missing dye bands. However, using standard testing guidelines and procedures, none of the 97 irradiated inks tested were found to show any significant optical or chemical differences from the control samples. In addition, random testing of some of the ink samples using a second solvent system did not reveal any changes. However, one control ink did show some minor changes in optical properties and dye characteristics over time (but not TLC) while the irradiated sample remained stable. Significant changes in the ultraviolet fluorescence characteristics of the irradiated paper samples themselves (not inks) were also observed.

The effect of electron beam irradiation on forensic evidence. 1. Latent print recovery on porous and non-porous surfaces.

The recent use of the postal system as a means of delivering anthrax spores via several contaminated envelopes has led to the selective irradiation of mail. These as yet unsolved attacks and the U.S. Postal Service's decision to irradiate certain types of mail has led to some unexpected complications. The high doses of radiation required to destroy biological agents like anthrax are sufficient to induce damage to other materials present in the envelope. There have been reports of damage to many different items that have been subjected to irradiation, including paper, precious gems, plastic, computer discs, and electronics. However, few studies have examined the effect of such treatments on items of forensic interest. In this paper, the authors focused on the impact of the irradiation process on the ability to visualize latent prints. This experiment involved using several donors, substrates (both porous and non-porous), and visualization reagents. The results indicate that the irradiation process can have a detrimental effect on the success of certain visualization reagents.

The forensic analysis of thermal transfer printing.

Thermal transfer printing refers to printing processes that utilize heat to produce an image by either physical or chemical means or by a combination of both. As the technology has improved and the supplies have become less expensive, the use of thermal printing in the personal and business markets has increased significantly. Specifically, dye diffusion thermal transfer and thermal mass transfer have become predominant in the production of counterfeit credit cards, drivers' licenses, and other types of documents produced on plastic media. Chemical analysis by means of thin layer chromatography (TLC) has proven to be useful in characterizing various types of inks (e.g., writing and inkjet inks). In this study, the authors examined 81 different samples that included a total of 54 printer samples (43 photographic prints on paper and eleven plastic card samples) and 27 printer ribbons. A new TLC method was developed and tested utilizing a solvent system (80% n-hexane, 3% methyl ethyl ketone, and 17% ethyl acetate) that is capable of producing excellent resolution.

The effects of latent print processing on questioned documents produced by office machine systems utilizing inkjet technology and toner.

Counterfeiting of currency and identity documents, death threats, illegitimate business transactions, and terrorist-related activities are some examples of the types of crimes that often involve documents produced from printers and copiers. Although standard protocol typically requires a questioned document (QD) examination prior to latent print (LP) processing, occasionally, items of evidence may be submitted for a QD examination following the application of a series chemicals utilized in the development of latent fingerprints. In such cases, the forensic examiner must take into account any previous treatments prior to initiating an examination on documents produced with a printer or copier. This study was devised to examine the effects of a latent print development technique [ninhydrin, physical developer, and a bleach enhancer] on the physical and chemical examination of documents produced from copiers and printers.